Composite

Part:BBa_K1893022

Designed by: Akash Bhattacharjee, Henry Lloyd-Laney   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 15:14, 27 October 2016 by AkashB 10 (Talk | contribs)


Rhl receiver with STAR (RhlR+pRhl+STAR)

This is the STAR sequence under control of the Rhl quorum signalling system. The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
Parameters
None