Coding

Part:BBa_K2043009

Designed by: Mislav Acman   Group: iGEM16_Paris_Bettencourt   (2016-10-13)
Revision as of 22:56, 26 October 2016 by Macman (Talk | contribs)

bpuI-FBD10 laccase codon optimized for E.coli

Description

The bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 10 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method. FBD10 is the first of 40 short peptides with affinity for cotton, linen, wool, polyester or silk identified in the Frank&Stain project. FBD10 is reported to have binding affinity for cotton (you can read more on this in the paper by. Unfortunately, this BioBrick was not tested for its activity due to a lack of time in iGEM competition. However, bpul and FBD1 specifications can be found on their BioBrick pages


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None