Composite

Part:BBa_K1893016:Design

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 07:04, 26 October 2016 by HOLL579 (Talk | contribs) (→‎Source)


Arabinose inducible gp2 (pBAD+gp2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1473
    Illegal SapI site found at 1098


Design Notes

Lots of stuff


Source

Gp2 was synthesised with a ribosome binding site using the ThermoFisher GeneArt service. This basic part (BBa_K1893019) was ligated in front of our pBAD construct BBa_K1893015

References