Generator

Part:BBa_I739002

Designed by: Christian Kemmer, Stefan Luzi   Group: iGEM07_ETHZ   (2007-09-27)
Revision as of 16:30, 27 September 2007 by Registry (Talk | contribs)

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Constitutive expression cassette for LacI +LVA (J23100.B0034.C0012.B0015)

The Biobrick encodes lacI (BBa_C0012) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None