Coding

Part:BBa_K1972000

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-09-16)
Revision as of 14:19, 25 October 2016 by Msshang (Talk | contribs)


dszA (a monooxygenase gene )

DszA is a monoxygenase which can convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszA require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 583
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1087


Figure 1：

T--SCUT-China A--u0.png

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Parameters
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