Composite

Part:BBa_K1954007:Design

Designed by: Kamil Żmijewski, Luba Prout   Group: iGEM16_UCL   (2016-10-19)
Revision as of 14:17, 25 October 2016 by Zcbtkjz (Talk | contribs) (→‎Design Notes)


Green Light Inducible bacteriocin Device (GLID)
In our design (Fig. 1), there are two independently inducible promoters (Ptac and pBAD) allowing for fine tuning of gene expression by modifying the amount of IPTG and arabinose. This device contains an improvement of the existing part of the device (BBa_K592019) because it constitutes a self-contained functional system with an improved RBS for PcpcG2 adapted from (1), suspected to increase the level of expression of the genes downstream of the promoter by about 40 times compared to the wild-type promoter.

Fig. 1. Green light-inducible device concept diagram.


References

  1. Abe K, Miyake K, Nakamura M, Kojima K, Ferri S, Ikebukuro K, et al. Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803: Engineered green light gene expression system. Microb Biotechnol. 2014 Mar;7(2):177–83.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1829
    Illegal NheI site found at 3697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3637
    Illegal BamHI site found at 4125
    Illegal XhoI site found at 3475
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943
    Illegal SapI site found at 3566


Design Notes

Source

To be added

References