Composite

Part:BBa_K1954007

Designed by: Kamil Żmijewski, Luba Prout   Group: iGEM16_UCL   (2016-10-19)
Revision as of 13:25, 25 October 2016 by Zcbtkjz (Talk | contribs)


Green Light Inducible bacteriocin Device (GLID) We designed a ‘template’ green light-inducible device (GLID) with blue output (amilGFP), adapted from Synechocystis sp. PCC6803. There are three components of the system: the chromatic acclimation sensor (CcaS), the response regulator (CcaR) and the PcpcG2 promoter. CcaS is a histidine kinase which is activated by green light and can catalyze phosphorylation of CcaR, leading to its activation and induction of gene expression downstream of PcpcG2.


In our design (Fig. 1), there are two independently inducible promoters (Ptac and pBAD) allowing for fine tuning of gene expression by modifying the amount of IPTG and arabinose. This device contains an improvement of the existing part of the device (BBa_K592019) because it constitutes a self-contained functional system with an improved RBS for PcpcG2 adapted from (16), suspected to increase the level of expression of the genes downstream of the promoter by about 40 times compared to the wild-type promoter.

Fig. 1. Green light-inducible device concept diagram.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1829
    Illegal NheI site found at 3697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3637
    Illegal BamHI site found at 4125
    Illegal XhoI site found at 3475
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943
    Illegal SapI site found at 3566


[edit]
Categories
Parameters
None