Intermediate

Part:BBa_K1976050

Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 11:20, 25 October 2016 by Jonassindlinger (Talk | contribs) (→‎Usage)

Colicin E2 DNase domain with B0034 RBS

Colicin DNase domain BBa_K1976048 with a strong RBSBBa_B0034.

Figure 1:Colicin E2 DNase domain based on an MD simulation.


Usage

Intermediate for assembly of generators of the Colicin E2 DNase domain.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007

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Parameters
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