Part:BBa_K1362011

RBS + λ-Lysozyme

This part, "linear" lysozyme, served as a control in a large thermostability screening of circular lysozme variants. Lysozyme is an enzyme that digests the peptidoglycan of bacterial cell walls.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

iGEM TecCEM lisozyme characterization <a href="https://static.igem.org/mediawiki/2016/0/02/T--TecCEM--16%29_caracterizacion_.pdf">[Characterization PDF]</a> essay was performed. A culture of E. coli DH5a cells transformed with BBa_J04450 biobrick was inoculated, so a red color would be a clear indicator of growth. Once the culture reached a certain OD several aliquots were taken. Each aliquot was mixed and incubated with a different quantity of proteic extract from the lysozyme protein. After some hours of incubation, said mixtures were tested for their optical density, so the lysis activity could be observed. So the less the optical density the measure resulted, the more lytic activity the lysozyme had on the cells.