Composite

Part:BBa_K1916100

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-09)
Revision as of 06:35, 25 October 2016 by Jkchen (Talk | contribs)


Ara-Inducible NpF2164g5 Expression System

A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain fused to the C-terminus for purification. Expression regulated by a pBAD promoter. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and cells appear vibrant blue-green under normal light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal SpeI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
    Illegal NgoMIV site found at 1182
    Illegal AgeI site found at 1272
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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