Intermediate

Part:BBa_K2036008

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 05:24, 25 October 2016 by ZhiZeng (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


RBS-CIII-RBS-CIII,tandem expression

As Ftsh normally exists in E.coli to degrade CII, CIII functions as an inhibitor of Ftsh, and tandem expression of CIII can repress the effect of Ftsh.

Protein&protein reaction

We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were constructed to test whether CIII can protect CII from being degraded by Ftsh by competitive inhibition.


Fig1: According to the Fluorescence measurement curve above, we can see clearly that GFP level of the CIII test circuit increases over time and it shows significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None