Device

Part:BBa_K1980011:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 21:05, 24 October 2016 by Sgarforth (Talk | contribs) (→‎Source)


pCopA MymT with divergent expressed CueR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 438
    Illegal NheI site found at 461
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli.

CueR is expressed divergent form the pCopA promoter.

Source

The source organism for the MymT protein sequence is Mycobacterium tuberculosis. The CueR and pCopA components of the promoter originate from E. coli.

We ordered this part as codon optimised DNA from IDT.

References