Coding
Part:BBa_K2130013
Designed by: Ooi Kean Hean Group: iGEM16_NTU-Singapore (2016-10-24)
SpCas9
This past is a human condon optimised SpCas9. SpCas9 recognises an 5'-NGG-3' PAM at the 3' end of the target sequence.
Example(PAM in brackets): 5'-NNNN...NNN(NGG)-3'
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 341
Illegal BglII site found at 1136
Illegal BamHI site found at 1430
Illegal XhoI site found at 1936 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1168
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 259
Illegal BsaI.rc site found at 1501
Illegal BsaI.rc site found at 2857
Illegal BsaI.rc site found at 3280
Illegal BsaI.rc site found at 3292
Illegal BsaI.rc site found at 4159
Illegal SapI.rc site found at 2964
Illegal SapI.rc site found at 3546
Illegal SapI.rc site found at 3561
[edit]
Categories
Parameters
None |