Coding

Part:BBa_K2130007

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-19)
Revision as of 13:56, 24 October 2016 by OOIK0003 (Talk | contribs)

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AsCpf1

Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)

Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal BglII site found at 1949
    Illegal BglII site found at 2258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3493
    Illegal NgoMIV site found at 3954
    Illegal NgoMIV site found at 3973
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None