Coding

Part:BBa_K2043006:Design

Designed by: Alicia Calvo-Villamanan y Sebastian Sosa-Carrilo   Group: iGEM16_Paris_Bettencourt   (2016-10-13)
Revision as of 12:21, 24 October 2016 by Calvo (Talk | contribs)


xylE-FBD10 from Pseudomonas putida codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 703
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag added to the C-terminal for optimised purification.

Source

Pseudomonas putida

References