Composite
c1

Part:BBa_K2027004:Design

Designed by: Charles Gleason   Group: iGEM16_Stanford-Brown   (2016-09-07)
Revision as of 10:12, 24 October 2016 by Htale1 (Talk | contribs) (→‎Design Notes)


Bacterial Collagen with Elastin Cross-linker and Coiled-Coil Heterotrimerization Domain 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI.rc site found at 785
    Illegal SapI site found at 175
    Illegal SapI.rc site found at 70


Design Notes

Below is the forward and reverse sequence verification of the construct, showing the elastin cross-linking domain, respective heterotrimeric coiled-coil, and bacterial collagen domain.

T--Stanford-Brown--c1seqver.png

Source

To be added before 2016 deadline.

References