Part:BBa_K2027044:Design

Bacterial Collagen with Coiled-Coil Heterotrimerization Domain 1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 75
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 519
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 72
  Illegal BsaI.rc site found at 65
  

Design Notes

Below is the forward and reverse sequencing verification for the part, showing that the digestion left the heterotrimeric coiled coil and bacterial collagen domain intact. <img src=>

Source

TBA