Coding

Part:BBa_K1976048

Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 16:35, 23 October 2016 by Jonassindlinger (Talk | contribs)

Colicin E2 immunity protein

This part encodes for the wild-type immunity protein inhibiting the nicking DNase Colicin E2. According to molecular dynamics simulations this protein inhibits also miniColicin (BBa_K1976048 and BBa_K1976049).

Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.


Usage

The Colicin E2 immunity protein can be used in combination with the Colicin E2 DNase or its engineered truncated variant miniColicin (BBa_K1976048 and BBa_K1976049).

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007

[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None