Regulatory

Part:BBa_K2180003

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-01)
Revision as of 15:28, 23 October 2016 by BenjaminBacri (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Sucrose Operator - DegU Binding Site

The sequence of the regulatory region comes from this site
We added two restriction sites SacII before and PacI after the regulatory region in order to use it in our project.
They allow us to replace a regulatory region with another regulatory region.
The regulatory region is composed by two DegU binding (one forward, one reverse) sites which are derepressed in the presence of sucrose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None