Composite

Part:BBa_K1893017

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 14:48, 23 October 2016 by HOLL579 (Talk | contribs) (→‎Functional Parameters)


Arabinose inducible GFP (pBAD+GFP)

A GFP reporter under control of a pBAD promoter, used to characterise the activation range of the pBAD promoter

Usage and Biology

Without any arabinose in the cell, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by the RNAP holoenzyme. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of GFP.

We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded fluorescence in a BMG plate reader.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2017
    Illegal SapI site found at 1098


<!>

Functional Parameters

[edit]
Categories
Parameters
None