Part:BBa_K1963010:Design
An sRNA system for down-regulation of E. coli fliC encoding flagellin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Design Notes
This part is RFC[10] compliant
Source
This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.
References
Erhardt M. (2016) Strategies to Block Bacterial Pathogenesis by Interference with Motility and Chemotaxis. Curr Top Microbiol Immunol [Epub ahead of print] doi:10.1007/82_2016_493
De Maayer P, Cowan DA. (2016) Flashy flagella: flagellin modification is relatively common and highly versatile among the Enterobacteriaceae. BMC Genomics 17:377. doi: 10.1186/s12864-016-2735-x.
Pushkin DO, Bees MA. (2016) Bugs on a Slippery Plane : Understanding the Motility of Microbial Pathogens with Mathematical Modelling. Adv Exp Med Biol 915:193-205. doi: 10.1007/978-3-319-32189-9_12.
Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols 8, 1694–1707. doi:10.1038/nprot.2013.105