RNA

Part:BBa_K1963010:Design

Designed by: Fatima Ulhuq   Group: iGEM16_Dundee_Schools   (2016-10-14)
Revision as of 13:16, 23 October 2016 by Franksargent (Talk | contribs) (→‎References)


An sRNA system for down-regulation of E. coli fliC encoding flagellin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

This part is RFC[10] compliant


Source

This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.

References

De Maayer P, Cowan DA. (2016) Flashy flagella: flagellin modification is relatively common and highly versatile among the Enterobacteriaceae. BMC Genomics 17:377. doi: 10.1186/s12864-016-2735-x.

Pushkin DO, Bees MA. (2016) Bugs on a Slippery Plane : Understanding the Motility of Microbial Pathogens with Mathematical Modelling. Adv Exp Med Biol 915:193-205. doi: 10.1007/978-3-319-32189-9_12.