Composite

Part:BBa_K1893011:Design

Designed by: Akash Bhattacharjee, Gear Rotrattanadumrong   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 11:29, 23 October 2016 by AkashB 10 (Talk | contribs)


Constitutive anti-STAR (J23119+anti-STAR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.

Source

Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time. It is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.

References

Imperial iGEM 2016