Part:BBa_K1963007
Device for rhamnose-dependent expression of an OsmY.
An assembled device that contains a rhamnose-responsive promoter, a strong RBS and a biobrick encoding the E. coli OsmY protein.
Usage and Biology
The E. coli OsmY protein is really interesting. Originally dubbed "osmotically-induced protein Y" it is synthesised with an N-terminal signal peptide (MTMTRLKISKTLLAVMLTSAVATGSAYA) most likely of the Sec type. While the protein has been reported to be periplasmic, it also appears to be one of the few fully secreted proteins that E. coli K-12 synthesises. This property has been used in SynBio projects (e.g. Steen et al. 2010) and in protein production projects (e.g. Le & Wang 2014).
An OsmY-encoding biobrick was assembled by Washington iGEM in 2012 (
Figure 1: Whole cell (A) and supernatant blots (B) of prhamnose-OsmY-HA (C) in the presence of increasing concentrations of Rhamnose. Anti-HA immunblot shows whole cell expression and secretion of OsmY-HA in the presence of Rhamnose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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