Composite

Part:BBa_K1961008:Design

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-14)
Revision as of 08:23, 23 October 2016 by Jonathan0912 (Talk | contribs) (→‎References)

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RecF protein generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 293
    Illegal SapI.rc site found at 459


Design Notes

No


Source

We ligased K1961001 with the existing part of BBa_K081005

References

Naofumi Handa, Katsumi Morimatsu, Susan T. Lovett, Stephen C. Kowalczykowski. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. GENES & DEVELOPMENT (2009). 23(10):1234–1245