Generator

Part:BBa_K1961004:Design

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-03)
Revision as of 06:38, 23 October 2016 by Jonathan0912 (Talk | contribs) (→‎Design Notes)


PbrR generator expressed by GFP under autoregulation of PTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2040


Design Notes

This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription.

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Source

We use parts in the 2016 iGEM plate R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012

References