Composite

Part:BBa_K2120401

Designed by: Lirong Zhao   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 06:11, 23 October 2016 by Beaz (Talk | contribs)


B0015+tetR+B0034+J23119+pTet+B0032+mazF+B0015

<Description>
We select the strong constitutive promoter J23119 and strong RBS B0034 to express inhibitor of pTet, tetR. And we ligate induced promoter pTet following the inhibitor. In order to decrease impact between constitutive promoter and induced promoter, we reserve the constitutive promoter’s direction and the transcription of it will not impact the downstream killer. Then killer, mazF, which locates at downstream of induced promoter will be regulated by concentration of inhibitor connecting plasmid copy numbers in a suitable range. Then this device can kill the cell with little plasmids and maintain a high expression level of function gene in industrial production.


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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NheI site found at 874
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None