Regulatory

Part:BBa_K1996000

Designed by: Claudia Moratti   Group: iGEM16_Sydney_Australia   (2016-10-14)
Revision as of 00:27, 23 October 2016 by Sche5991 (Talk | contribs)


EtnP-Putative promoter region for ethylene oxidation genes in Mycobacterium NBB4

Putative promoter for suspected ethylene-oxidising genes sourced from Mycobacterium NBB4

Usage and Biology

This part was obtained from the ethylene metabolism operon in Mycobacterium NBB4. It is thought to be a promoter that regulates bidirectional transcription of gene involved in ethylene metabolism (Figure 1). The enzymes required for ethylene metabolism are contained within an operon organised in two bidirectional gene clusters. One gene cluster encodes the catabolic enzymes required to convert ethylene into acetyl-CoA, the final product of the degradation pathway which is subsequently directed to the tricarboxylic acid cycle. The other gene cluster encodes the enzymes responsible for synthesising coenzyme M, a cofactor required for the function of the second enzyme in the metabolic pathway.

T--Sydney_Australia--gene_cluster.png
Figure 1. Schematic of the ethylene metabolism operon in Mycobacterium NBB4. The two bidirectional gene clusters are indicated by the curved brackets. Between the two clusters is the putative promoter for their transcription, EtnP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None