Composite
Part:BBa_K1963008
Designed by: Frank Sargent Group: iGEM16_Dundee_Schools (2016-10-09)
Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..
A composite of three biobricks to give rhamnose-regulated expression of an sRNA secretion system - a translational fusion between E. coli OsmY and E. coli Hfq.
Usage and Biology
Experimental procedures needed.
Figure 1. Whole cell blots expressing OsmY-Hfq (E.coli) fusion protein under the control of the rhamnose promoter.
Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/composite_part/nominee
//awards/part_collection/2016
//awards/part_collection/2016
None |