Composite

Part:BBa_K1983017:Design

Designed by: Vykintas Jaunikis   Group: iGEM16_Vilnius-Lithuania   (2016-10-13)
Revision as of 21:41, 22 October 2016 by Vykintas (Talk | contribs) (→‎Source)

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PheP under constitutive promoter, medium strength RBS and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 98
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG

Primers used for colony PCR screening:

For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc

Primers used for cloning a medium strength RBS:
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC
RBS-Med: GCATCTAGAGACTCTAATCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG

Source

This part is derived from (BBa_B0017). Other parts are derived Escherichia coli and were synthesized by Integrate DNA Technologies.

References