Device

Part:BBa_I746313

Designed by: Cambridge iGEM 2007 team   Group: iGEM07_Cambridge   (2007-09-13)
Revision as of 08:30, 13 September 2007 by Stefan (Talk | contribs)

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arabinose induced expression of P2 ogr and mRFP

In this device the arabinose induced pBAD/araC promoter controls expression of the P2 ogr activator and mRFP. It is used to induce P2 ogr expression.

The P2 ogr (I746350) does contain a RBS (B0034)!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2036
    Illegal AgeI site found at 2148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None