Composite

Part:BBa_K1893003:Design

Designed by: Alyssa Henderson, Aditi Satija   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 19:14, 22 October 2016 by Ach196 (Talk | contribs)


Rhl receiver with GFP reporter (RhlR+pRhl+GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776
    Illegal BsaI.rc site found at 1658


Design Notes

This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the RhlR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pRhl promoter, which is, in this case, GFPmut3b.


Source

Freemont Lab, Imperial College London

References