Composite

Part:BBa_K1962010

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-12)
Revision as of 18:53, 22 October 2016 by Frulhuq (Talk | contribs)


Bile Salts Sensing Device

This is a composite part which consists of the bile salt responsive promoter (BBa_K318514) and a combined ribosome binding site / GFP / terminators biobrick (BBa_E0840). The acrRA operon is found in Salmonella enterica strain LT2 and the promoter sequence contains a RamA (BBa_K1962009) binding sequence.

Usage and Biology

T--Dundee--GreenResults2.png

File-T--Dundee--Resultsfrulhuq18.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal XhoI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1508


[edit]
Categories
Parameters
None