Part:BBa_K1983015
PheP and tRNA-Phe under constitutive promoters
Overview
This biobrick part contains two coding elements: (BBa_K1983014): PheP, which is labeled with N-6XHis-Tag and put under a constitutive promoter (BBa_K823005), high strength RBS 9BBa_J61132) and terminator (BBa_B0017). A basic biobrick part (BBa_K1983011): tRNA-Phe (pheU) under constitutive promoter and terminatorPheP (Phenylalanine-specific permease) is a natural phenylalanine membrane transporter of the bacterium Escherichia coli.
PheP is a single integral membrane transporter which selectively transports L-phenylalanine and L-tyrosine as an antiporter using proton motive force. The activity of this transporter under natural expression is known to be 9 and 17,5 nmol/mgDW(cells) for L-phenylalanine and L-tyrosine respectively [1].
pheU gene from Escherichia coli encodes a tRNA transporting L-phenylalanine and is under an upstream constitutive promoter and a terminator found after the tRNA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 98
Illegal NheI site found at 1669
Illegal NheI site found at 1692 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |