Device

Part:BBa_K1954002

Designed by: Yuqiao Liu, Kamil Żmijewski   Group: iGEM16_UCL   (2016-10-14)
Revision as of 12:15, 22 October 2016 by LubaProut (Talk | contribs)


pGAP-Vitellogenin-GFP device

A previous BioBrick has been designed to detect misfolding of proteins in the periplasm has been found to be sensitive to high pH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1905
    Illegal NheI site found at 2139
    Illegal NheI site found at 4457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 3274
    Illegal BglII site found at 3900
    Illegal BglII site found at 3936
    Illegal BamHI site found at 1281
    Illegal XhoI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 806
    Illegal BsaI site found at 3681
    Illegal BsaI site found at 3811
    Illegal SapI site found at 244
    Illegal SapI site found at 3868
    Illegal SapI.rc site found at 5732

Usage and Biology

[edit]
Categories
Parameters
None