Coding
OMT-RS

Part:BBa_K1976020

Designed by: Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Schäfer, Inka Schröter, Viktoria Schuster   Group: iGEM16_TU_Darmstadt   (2016-10-13)
Revision as of 10:21, 22 October 2016 by SabineSch90 (Talk | contribs)

O-Methyl-L-tyrosine tRNA synthetase

This Part encodes the tRNA for nonnatural aminoacid O-Methyl-L-Tyrosine and its synthetase.

Figure 1:Dimer of the Methanocaldococcus jannaschii tyrosyl-tRNA synthetase specific for O-methyl-l-tyrosine


Usage

The orthogonal pair for amber supression consists of the tRNA with an anticodon complementary to the amber stop codon and an aminoacyl RNA synthetase (BBa_K1976021) specifically loading the tRNA with the non-natural amino acid. The occurence of O-methyl-L-tyrosine does not stop the translation of the immunoprotein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[edit]
Categories
Parameters
None