Coding

Part:BBa_K2074026

Designed by: Hao Feng   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 09:55, 22 October 2016 by Heao (Talk | contribs)


EGFP(Codon optimization) 

 Our part is coding the EGFP,We transferred the gene EGFP into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein and to test whether our systerm work or not.
 We used the promoter to initiate expression of EGFP, resulting in promoter regulation of gene expression data

 The efficiency of Hsp70A-Rbc S2 promoter can be improved when environmental temperature is larger than 40℃. It means that the toxins express device will express more toxins in summer closed waters.The closed waters usually is larvae living place. The temperature in the open water will be much lower than this value. This decided would reduce the amount of pro-toxic protein present in the non-functioning waters.
T--FAFU-CHINA--Result4.png
T--FAFU-CHINA--Result5.png
T--FAFU-CHINA--Result6.png


T--FAFU-CHINA--EGFPoptimization.png
T--FAFU-CHINA--EGFPoptimization2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]
File:T--FAFU-CHINA--E.png
Figure.1 The gel electrophoresis result of EGFP
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Categories
Parameters
None