Coding
Part:BBa_K2130000
Designed by: Ooi Kean Hean Group: iGEM16_NTU-Singapore (2016-10-19)
∆RuvCIII-2 ∆HNH Sp-dCas9
dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2350
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2496
Illegal BsaI site found at 3158
Illegal BsaI.rc site found at 1411
[edit]
Categories
Parameters
//awards/basic_part/nominee
None |