Coding

Part:BBa_K2074026

Designed by: Hao Feng   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 03:39, 22 October 2016 by Heao (Talk | contribs)


EGFP(Codon optimization)

our part is coding the EGFPE,We transferred the gene EGFP into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein and to test whether our systerm work or not.

800px-T--FAFU-CHINA--Result4.png 800px-T--FAFU-CHINA--Result5.png


T--FAFU-CHINA--EGFPoptimization.png

T--FAFU-CHINA--EGFPoptimization2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]
File:T--FAFU-CHINA--E.png
Figure.1 The gel electrophoresis result of EGFP
[edit]
Categories
Parameters
None