Reporter

Part:BBa_K1943019:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-10-13)
Revision as of 03:16, 22 October 2016 by John Tsq (Talk | contribs) (→‎Source)


TetOn


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857


Design Notes

This TetOn expression system contains a SV40 promoter, a puro anti-antibiotics gene, 2A and TetOn 3G promoter. We design primers and do PCR of this TetOn expression system and ligate it to pSB1C3 backbone.

Source

The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.

References