Part:BBa_K1729002

Yeast MRPS12 mitochondrial localization signal

This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of Saccharomyces cerevisiae mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview MRPS12]. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda et al.).

The part sequence corresponds to nucleotides 694840-694739 on chromosome XIV of the S. cerevisiae S288C reference genome. See also BBa_K1729001

Screen capture from SnapGene®

References

Branda, Steven S et al. "Prediction and Identification of New Natural Substrates of the Yeast Mitochondrial Intermediate Peptidase." J Biol Chem 270 (1995): 27366-73.

Uses

Used in part BBa_K2077002. When connected to a fluorescent protein by a GS linker, mls targets fluorescence to yeast mitochondria even in strains with defunct mitochondria lacking ribosomal protein mRPS12, which is necessary for yeast mitochondria to translate their genome and make electron transport proteins needed to generate a membrane potential.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]