Part:BBa_K1954002:Design
pGAP-Vitellogenin-GFP device
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1905
Illegal NheI site found at 2139
Illegal NheI site found at 4457 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 3274
Illegal BglII site found at 3900
Illegal BglII site found at 3936
Illegal BamHI site found at 1281
Illegal XhoI site found at 577 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 806
Illegal BsaI site found at 3681
Illegal BsaI site found at 3811
Illegal SapI site found at 244
Illegal SapI site found at 3868
Illegal SapI.rc site found at 5732
Design Notes
Mutagenesis of UCL iGEM 2009 BBa_K239009.
The following is the sequence of Vitellogenin proposal sequence:
gaattcgcggccgcttctagagagatcttttttgtagaaatgtcttggtgtcctcgtccaatcaggtagccatctctgaaatatctggctccgttgcaactccgaacgacctgctggcaacgtaaaattctccggggtaaaacttaaatgtggagtaatggaaccagaaacgtctcttcccttctctctccttccaccgcccgttaccgtccctaggaaattttactctgctggagagcttcttctacggcccccttgcagcaatgctcttcccagcattacgttgcgggtaaaacggaggtcgtgtacccgacctagcagcccagggatggaaaagtcccggccgtcgctggcaataatagcgggcggacgcatgtcatgagattattggaaaccaccagaatcgaatataaaaggcgaacacctttcccaattttggtttctcctgacccaaagactttaaatttaatttatttgtccctatttcaatcaattgaacaactaatacaaatgttggccccaggattcgcctctgttaagacctacatgtacaaatatgaagcggttcttatgggcggcctgcctgaagagggcctggctcgagctggggttaaaatccggggcaaagttttgatcagtgcaacaagtgccaacgactacattctgaagcttgtagaccctcagttgctggagtacagtggcatctggcccaaagatcctttccatccagccaccaagctcaccacagccctggctactcagctctcgacaccgatcaagtttgagtatacaaacggcgttgtcgggagactggctgcacctcctggggtctccacaacagtgctgaatatctacaggggaatcatcaacctcctgcaactgaatgtaaagaagacacagaacgtctacgagatgcaagagtctggagctcatggtgtgtgcaagaccaactatgtgatcagggaggacgcgagagccgaacgcattcatctgaccaagaccaaggacctgaaccactgccaggagaaaatcatgaaggccatcggcttggaacacgtagagaaatgccatgattgtgaagctagaggaaagagcctgaagggaactgcttcctataactacatcatgaagccagcacccagtggttctctgattatggaggctgtcgctagagaggtcatcgagttctcacctttcaacattttgaatggcgctgctcagatggagtctaagcaaattctgaccttcctggatatcgagaacacccctgtggatcatgccagatacacctatgttcaccgcggatccctgcaatatgagcatggcagcgagattctccagacacccatccatcttctgagggtcacccatgccgaggctcagattgtcagcactctgaaccacctggtagcctccaacgtggccaaggtccatgaagatgcccctctgaagtttgttgagctcatccaggtgatgcgtgtggccagatttgagactattgagtccctctgggctcagtttaaatccagacctgatcacaggtactggttactgaatgctgtcccccacattcgcactcacgctgcgcttaagttcctcattgagaagctccttgctaatgagttaagtgagactgaagctgctatggctctcttggaatgtctgcactctgtgacagctgaccagaaaaccattgaacttgtcagaagcctggctgagaaccacagagtgaaacgtaacgctgtgctcaacgagattgtgatgctgggctggggcactgtaatttccaggttctgtaaagcgcagccatcttgctcatctgatcttgtgacacctgtacatagacaagttgcagaggctgttgaaactggtgacatcgatcagctcactgtcacgctcaaatgcctggataacgctggacatcctgctagcattaagacaatcatgaagttcctgcctggctttggcagtgctgctgcccgagtcccactcaaagttcaggttgacgctgttctagccctgaggagaattgcaaagagggaacccaagatggtccaggaaatagctgctcagttgctcatggaaaagcatctccatgcagaactgcgtatggttgctgccatggtgctctttgagactaaactccccgtgggtctagcagctagcatttccacagccttgatcaaagaaaagaacctgcaagtcgttagctttgtctactcttacatgaaggccatggccaagaccacatcccctgaccacgtttctgttgctgccgcatgtaatgttgccttgaggttcctcaaccccaaattaggcagactgaacttccgctacagccgagccttccatgtggatacctataacaatgcctggatgatgggtgctgccgccagtgccgtcttaattaacgacgctgcaaccgtgttaccaagaatgattatggccaaagcccgtacttacatggccggagcttatgttgatgcttttgaggttggagtgaggactgagggaatccaggaggctcttttgaaaagacgacatgaaaattctgagaatgcagacaggatcaccaagattaaacaagccatgagagctctttctgagtggagggctaatccttcgagccaggccctggcctctatgtatgtgaaggtcttcggacaagaaattgcatttgccaacattgacaaatccaaggttgaccagcttatccagtttgccagtggacctttgagaaacgtattcagagatgctgtgaactctgtgctgtctggttatgcaacacattttgctaaaccaatgctgctcggtgagctccgtctcatccttcccaccactgttgggttgcccatggagatcagcctcattacatccgctgtgactgctgcatctgttgacgtccaagccactgtgtcaccacctctgcctgtcaactaccgagtttcccagcttctggagtccgatatccaactgagggctacagttgctccaagtcttgccatgcagacctatgcattcatgggtgtgaacaccgccttaatccaggctgccgtgatgacaaaagccaaagtttacacagctgttcctgcacagataaaagcaaggattgacattgttaagggcaacttgaaggttgagttcctgtctctccagggcattaacacaattgcatctgcacatgcggagacggttgccattgcaagaaatgtggaagacctcccagccgcaagaagcacaccactgatctcatctgaaactgcatcacaactttcaaaggcctctctcaactcaaagatctccaggatggcatcctctgtgactggtggcatgtctgcgtcatctgaaatcattcctgctgacctgccaagtaagattgggaggaaaatgaaactccctaaaacctacaggaagaaaatccgtgcttcaagcagaatgctaggattcaaggcctacgctgagattgaatctcacaatgccgcctacatcagagactgccctctctacgctctgatcggaaagcatgctgcttctgttaggattgctccagcttctggaccagtcattgagaagattgaagttgagattcaggtcggagataaagcagcagaaaatatgattaaagcgattgacatgagcgaagaggaggaagctcttgaggataagaatgtcctcttgaaaatcaagaaaatactggcacctggtctcaagaacaccacatcatcttcctccagctcctccagctcctcttcatccagctctagctccaacaagtcttcttcatccagttcccgctccagcagctcccagtcatccagctctcgttcccataggtctcgctccagaaagtcccagtctagcagctctcagtcaagccgctctccctcaagctcttcctcctcttcctcctcttcatcatccagatcttcttccaggtcatcttccagatcatcttccagatcttcttctaggtcctcctctcgctccagaactaagatggctgacattgttgctcctattatcacgacgtccaccagagtgagcagttcctccagtcgatcagcctctaacagctcctccagcagtgcttcatacttgctcagctcatctaaaagaagaagcagaagcagaagcagcagcagcagtagcagcagcagcagcagcagcagcagtagcagcagcagcagcagcagcagtagcaagaacagcaagcgcagcaagagcagcaacagcaagtcatcaagctctaggtcctctcggcgcagtgctcagtctaagcaacaactgcttgccttgaagttcagaaagaaccacgtccacaggcatgccatctccacacagcgcggcagcagtcacagcagtgcccgcagcttcgattccatctacaataaggccaagtacctcgctaacacactcactcctgccatgtccattgcaatccgtgccgtgagagtcgaccacaaggtccagggataccagctagcagcttacctggacaaacagaccaatagactgcaattgatttttgccagagtcgctgagaaggacaactggagaatctgtgccgacattgtgcagctgagttcgcacaagctgatggccaagactgcctggggtgctgaatgcaagcaatactccaccatgattgtagctgaaactggtcttttgggtcatgagcccgcagcccgcttgaagctgacctgggacaaactgccaggaagcataaagcactacgcaaagagggcgttgaaatccattgtccctattgctcaagaatatggagtaaactacgcaaaggccaagaatcctcgtaatcaaatcaaactgactgtagctgttgctactgagacaagcatgaatattgtgctgaacacaccaaaggcaatcgtttacaagcgtggggtgtgtctacctgttgctttaccaattggaaacactgctgccgagctgcaagcgacccgggacaactgggctgacaagatgtcctatttggttaccaaagctaacgcagttgaatgctccctcatcaacaacacactgaccacattcaacaacaggaaagctagagatgagctgccacactcgtgctaccaggtcttggctcaggattgcacaccagaactcaaattcatggttctgctgaagaaagaccaaatacaggatcagaaccagatcaatgttaagatttcagacatcgatgtggacatgtatcggaagaacaacgccattgcggtgatggttaacggagttgaaatccctaacagcaacctgccatacctgcatccatcaggtaacatacatataagacagtcaaatgaaggcattactctcaatgcacccagccatggtcttcaggaggtcttccttggcttcaacgagctgagggttaaagttgcagactggatgaaaggaaagacttgtggtgcctgtggaacggcaagcggaaatgtcggagacgagtaccgcacacccagtgaacaggtgaccaaggatgccatcagctacgcccactcctgggttctgtcttcaaacacctgccgtgatccctccgagtgttccatcaagcaggaatctgtgaagctggagaagcgggtgatctttgaaggtgtggagtccaaatgctactctgttgagcccgtgctgcaatgcctgcccggctgtatcccagtgagaaccactaccgtcaacgttggctttcactgcctgcccagtgacacaactgtggaccgttctggtctgagcagcttctttgagaagagcatcgacctgagggatactgccgaagcccacctggcctgtcgctgcactcctcagtgtgctgagttcatggtttctaaaggtgaagaattgttcactggtgttgttccaattttggttgaattggatggtgatgttaatggtcataaattctctgtttctggtgaaggtgaaggtgatgctacttatggtaaattgactttgaaattcatttgtactactggtaaattgccagttccatggccaactttggttactactttgacttatggtgttcaatgtttctccagatatccagatcatatgaaacaacatgatttcttcaaatctgctatgccagaaggttatgttcaagaaagaactattttcttcaaagatgatggtaattataaaactagagctgaagttaaattcgaaggtgatactttggttaatagaattgaattgaaaggtattgatttcaaagaagatggtaatattttgggtcataaattggaatataattataattctcataatgtttatattatggctgataaacaaaaaaatggtattaaagttaatttcaaaattagacataatattgaagatggttctgttcaattggctgatcattatcaacaaaatactccaattggtgatggtccagttttgttgccagataatcattatttgtctactcaatctgctttgtctaaagatccaaatgaaaaaagagatcatatggttttgttggagttcgttactgctgctggtattactttgggtatggatgaattgtataaatctggtttgaggtccagagctcaagcttctaattctgctgttgatggtactgctggtccaggttctactggttccagagcttgtaaaaattggttctcttctttgtctcatttcgttattcatttgtaactactagtagcggccgctgcaggaattc
48% GC
Source
UCL iGEM 2009.