Coding

Part:BBa_I746200:Design

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-29)
Revision as of 10:52, 8 September 2007 by Narin (Talk | contribs) (→‎Source)


FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 238
    Illegal AgeI site found at 604
    Illegal AgeI site found at 1231
    Illegal AgeI site found at 1749
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.


Source

The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba. The main paper reference is:

Effect of loop deletions on the binding and transport of ferric enterobactin by FepA. Mol Microbiol. 1999 Jun;32(6):1153-65. - Newton SM, Igo JD, Scott DC, Klebba PE.

References