Coding
Part:BBa_K1983000:Design
Designed by: Vykintas Jaunikis Group: iGEM16_Vilnius-Lithuania (2016-08-01)
Codon optimized AvPAL with C-terminal 6XHis-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1265
Illegal XhoI site found at 1702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1483
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG
Primers used for colony PCR screening:
For pBAD:
Bad-P1: AGATTAGCGGATCCTACCTGACGC
Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC
For pETDuet-1:
Up-A1: GGATCTCGACGCTCTCCCT
Down3: ACCCCTCAAGACCCGTTTAG
Primers used for cloning into biobrick:
PAL-Chis-BBa-FW: AATCTAGatgaagaccctgtcccagg
PAL-Chis-BBa-RV: AAACTAGTATTAGTGATGGTGGTGGTGATG
Source
E coli