Coding

Part:BBa_K2127002:Design

Designed by: Dawson Zeng   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)
Revision as of 13:47, 21 October 2016 by Nhaddou (Talk | contribs) (→‎Source)


CP47 His-tagged with Lac Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 597
    Illegal AgeI site found at 1194
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918
    Illegal SapI site found at 1595


Design Notes

Double Stop Codon. ORF Check


Source

The psbB gene codes for the CP-47 subunit of the Cyanobacterial Photosystem II in Synechocystis sp. PCC 6803. Using site-directed Mutagenesis Dr Frankel’s team developed a Synechocystis sp. PCC 6803 mutant containing a histidine tag at the C-Terminus of CP47 subunit designated mutant HT-3.

NCBI Reference Sequence: NC_000911.1

References