Terminator
MaTif

Part:BBa_K2127007:Design

Designed by: Mirat Sojitra   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)
Revision as of 11:19, 21 October 2016 by Nhaddou (Talk | contribs) (→‎References)


MT - TERMINATOR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The terminator sequence is based off of the double terminater BBa_B0015. To ensure ability to synthesis in a gblock from IDT,nine base pairs were removed from the 3' end of the sequence. The effect in termination efficiency has not been analyzed. Further insight into the effect of the nine base pair would be beneficial to the Registry.

Source

The base sequence is taken from the BBa_B0015 part available on the IGEM registry submitted by Reshma Shetty. The double terminator contains both the BBa_B0010 and BBa_B0012. This template double terminator is highly characterized and commonly used. As mentioned, adjustments were made due to limitations in gBlock synthesis from IDT.

References

Shetty, R and Antiquity Team.(2007)BBa_B0015. IGEM Parts Registry. https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=BBa_B0015. Accessed: October 5 2016