Terminator
MaTif

Part:BBa_K2127007:Design

Designed by: Mirat Sojitra   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)
Revision as of 11:09, 21 October 2016 by Nhaddou (Talk | contribs) (→‎Design Notes)


MT - TERMINATOR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The terminator sequence is based off of the double terminater BBa_B0015. To ensure ability to synthesis in a gblock from IDT,nine base pairs were removed from the 3' end of the sequence. The effect in termination efficiency has not been analyzed. Further insight into the effect of the nine base pair would be beneficial to the Registry.

Source

MT - TERMINATOR

References