Composite

Part:BBa_K1992010

Designed by: Shilo Ohayon   Group: iGEM16_Technion_Israel   (2016-10-14)
Revision as of 10:33, 21 October 2016 by Shilo (Talk | contribs) (→‎Design considerations)

PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 331
    Illegal NgoMIV site found at 412
    Illegal NgoMIV site found at 416
    Illegal NgoMIV site found at 435
    Illegal AgeI site found at 535
    Illegal AgeI site found at 746
    Illegal AgeI site found at 1601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2657


Introduction

A GFP gene was fused to the PctA chimera. The plasmid is comprised of a promoter, a strong RBS, the chimera, a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein.


Usage and Biology

This expression device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane.


Experiments and results

For methods and results please go to PctA-Tar (BBa_K1992001) ot to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page



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