Part:BBa_K1996002
EtnR2 - Putative ethylene receptor in Mycobacterium NBB4
Putative ethylene receptor
Usage and Biology
EtnR2 is a native protein of Mycobacterium NBB4. This sequence has been codon-optimised for expression in E. coli. where expression has been achieved in a T7 system with pGro7 chaperone protein (Figure 1) with protein identity confirmed by mass spectrometry. EtnR2 is believed to be a regulatory protein that senses ethylene levels and phosphorylates EtnR1 protein (BBa_K1996001) which modulates expression of enzymes associated with ethylene metabolism. We suspect that together, EtnR1 and EtnR2 represent a two-component regulatory system responsible for activating gene expression from the putative promoter EtnP (BBa_K1996000) upon exposure to ethylene.
Figure 1. SDS-PAGE sizing of EtnR1 and EtnR2 expressed following 6 hour IPTG induction. Cellular lysates were isolated from BL21 (DE3) E. coli. with pGro7 chaperone plasmid expressing pET-EtnR1 or pET-EtnR2, and separated electrophoretically on 12% SDS-PAGE. The bands around 66-70 kDa and 25-28 kDa respectively were trypsin-digested and analysed using mass spectrometry. (a) Isolated EtnR1 lysates. (b) Isolated EtnR2 lysates.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 56
| None |