Device

Part:BBa_I746201

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-31)
Revision as of 10:03, 31 August 2007 by Registry (Talk | contribs)

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Outer Membrane Permeability Device

FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted. The L8T mutant is otherwise similar to the L8 mutant apart from the introduction of a beta turn at the deletion junction (NCET).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 299
    Illegal AgeI site found at 665
    Illegal AgeI site found at 1292
    Illegal AgeI site found at 1810
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None